منابع مشابه
Lipid bilayer vesicle generation using microfluidic jetting.
Bottom-up synthetic biology presents a novel approach for investigating and reconstituting biochemical systems and, potentially, minimal organisms. This emerging field engages engineers, chemists, biologists, and physicists to design and assemble basic biological components into complex, functioning systems from the bottom up. Such bottom-up systems could lead to the development of artificial c...
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Vesicle assembly in space and time Two talks used technological advances in light and electron microscopy to examine the formation of vesicles from the plasma membrane. In yeast, endocytosis proceeds via clathrin-tipped tubular invaginations. Andrea Picco (Kaksonen laboratory, European Molecular Biology Laboratory) used live-cell imaging to reconstruct the dynamic organization of the yeast endo...
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Traditional liposome preparation methods are based on mixing of bulk phases, leading to inhomogeneous chemical and/or mechanical conditions during formation; hence liposomes are often polydisperse in size and lamellarity. Here we show the formation of liposomes that encapsulate reagents in a continuous two-phase flow microfluidic network with precision control of size from 100 to 300 nm by mani...
متن کاملUnilamellar vesicle formation and encapsulation by microfluidic jetting.
Compartmentalization of biomolecules within lipid membranes is a fundamental requirement of living systems and an essential feature of many pharmaceutical therapies. However, applications of membrane-enclosed solutions of proteins, DNA, and other biologically active compounds have been limited by the difficulty of forming unilamellar vesicles with controlled contents in a repeatable manner. Her...
متن کاملDynamic pattern formation in a vesicle-generating microfluidic device.
Spatiotemporal pattern formation occurs in a variety of nonequilibrium physical and chemical systems. Here we show that a microfluidic device designed to produce reverse micelles can generate complex, ordered patterns as it is continuously operated far from thermodynamic equilibrium. Flow in a microfluidic system is usually simple-viscous effects dominate and the low Reynolds number leads to la...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2006
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac069424n